Diffuse large B-cell lymphoma in leukemic phase.

A 76-year-old man presented with right shoulder pain, weight loss, and night sweats. Complete blood count showed isolated thrombocytopenia (26 3 10/L). The blood film demonstrated medium to large atypical lymphoid cells (12% of leukocytes), many with polylobated nuclei or flower cell morphology (panel A, image obtained using Olympus BX51 microscope, 31000 magnification). Computerized tomography identified a right scapular mass and retroperitoneal lymphadenopathy. Bone marrow examination (panel B) and biopsy of the scapular mass (panel C) revealed infiltration with atypical lymphoid cells identical to those in peripheral blood. These cells expressed CD20 and cytoplasmic immunoglobulin M (IgM) and were negative for surface immunoglobulin, CD3, and CD34 on flow cytometry. Terminal deoxynucleotidyltransferase and c-myc were negative on immunohistochemistry. Ki-67 was 40%. Cytogenetics identified an abnormal karyotype: 47, XY, t(1;6)(q32;q21), t(3;14)(q27;q32), 1der(3)t(3:14). Fluorescence in situ hybridization confirmed B-cell lymphoma 6/IgH fusion at t(3;14). These features were consistent with diffuse large B-cell lymphoma in leukemic phase with flower cell morphology.